Biochemistry Essentials: Key Concepts and Q&A

Biochemistry: Key Concepts and Q&A

Metabolic Pathways and Cellular Respiration

  • Which of the following metabolic pathways does *not* happen inside the mitochondria? Acetyl-CoA production
  • Which of the following elements increase the activity of the pyruvate dehydrogenase complex (PDH)? All of them
  • The flow rate through the Krebs cycle is controlled by: All of them
  • Indicate the true statement about the respiratory chain and oxidative phosphorylation: In eukaryotes, oxidative phosphorylation occurs in mitochondria and involves the oxidation of O2 to H2O.
  • Which of these following types of molecules are able to accept and donate electrons: All the previous statements are true
  • Which of the following molecules is necessary to transport acetyl-CoA inside the mitochondria? Carnitine

Fatty Acid and Amino Acid Oxidation

  • Which of these combinations of reactions happen in the beta-oxidation of fatty acids? Oxidation, hydration, oxidation, thiolysis
  • When amino acids are deaminated in muscle, the produced ammonia is mostly… :
  • Indicate the false statement about amino acid oxidations:

DNA, Transcription, and Translation

  • If one molecule of DNA has 20% of A, how much does it have for the other nitrogenous bases, and how does its melting point behave compared to one molecule of 10% of A?
  • Which of the statements about chromosomes and their properties is false?
  • If DNA polymerase I was mutated to inactivate all its enzymatic activities, which part of DNA replication will be most affected?
  • Indicate the true statement about transcription:
  • Which of the statements is false about the poly(A) tail:
  • Indicate the false statement about translation:

Protein Structure and Function

  • Given this protein structure that you can see below, indicate which of the following statements is false: This protein is soluble in water as all globular proteins
  • Which of these statements is true about the structure of an aldehyde: An aldehyde contains a carbon atom bound to an oxygen atom by a double bond in the terminal part of the molecule
  • When is the buffering power of an acid-base mixture maximal: When pH is lower than pKa
  • Indicate the true statement about free energy: All the other statements are false

Enzymes and Carbohydrates

  • In which cell types is glycogen present in high concentration?
  • Indicate the term that can be defined as “a complete catalytically active enzyme together with its bound coenzyme and/or metal ions”: Holoenzymes
  • Which of the following factors can affect enzyme activity: All of them
  • Which of these carbohydrates present β-linkages in their structures:
  • Which of these variables can affect the quantity of vitamins required for a healthy diet: All of them

Glycogen Metabolism and Hormonal Regulation

  • Liver glycogen breakdown is stimulated by: Both (b) and (c) (Glucagon and Epinephrine)

Monosaccharides and Chemical Reactions

  • Indicate the name and the number of enantiomers of a monosaccharide of five carbon atoms that contains a carbonyl group in the first carbon and 3 chiral atoms:
  • Indicate the false statement about the ΔG value of a chemical reaction: It indicates the direction in which the reaction occurs spontaneously

Signal Transduction and Pentose Phosphate Pathway

  • Which of these features is *not* one from signal transductions? Adaptation
  • Indicate the false statement about the pentose phosphate pathway: It takes place only in the liver

Nucleotides

  • Which of these is *not* a function of nucleotides? Metabolic Fuels