Prokaryotes vs. Eukaryotes: Cell Structure, Function & Differences
Eukaryotes vs. Prokaryotes
- Organelles:
- Unique to Prokaryotes:
- Nucleoid: Region containing genetic material (DNA), lacks a membrane. Allows for rapid gene expression.
- Cell Wall: Provides structural support, protects against osmotic pressure changes, and helps maintain cell shape. Composition varies among bacteria (peptidoglycan in most).
- Plasmid: Small, circular DNA molecule separate from the chromosomal DNA. Often carries accessory genes, like antibiotic resistance. Facilitates horizontal gene transfer.
- Common in Bacteria:
- Flagella: Long, whip-like appendages enabling movement. Rotates like a propeller, allowing bacteria to swim toward favorable environments or away from harmful ones.
- Pilus/Fimbriae: Hair-like appendages facilitating attachment to surfaces or other cells. Important for biofilm formation and bacterial conjugation.
- Unique to Prokaryotes:
- Gene Transfer:
- Methods in Bacteria:
- Conjugation: Direct transfer of genetic material between bacterial cells through physical contact facilitated by sex pili. Enables exchange of plasmids, contributing to genetic diversity and antibiotic resistance.
- Transduction: Transfer of DNA from one bacterium to another by viruses (bacteriophages) that infect bacteria. Can introduce new genes into bacterial populations.
- Transformation: Uptake of foreign DNA from the environment, integrated into the bacterial genome. Allows bacteria to acquire new traits, such as antibiotic resistance.
- Methods in Bacteria:
- Staining and Shapes:
- Staining helps differentiate bacteria based on cell wall composition and morphology. Shapes (cocci, bacilli, spirilla) aid in identification and classification of bacteria.
- Antibiotics:
- Target specific bacterial structures or functions, such as cell wall synthesis, protein synthesis, or DNA replication.
- Exploit differences between bacterial and eukaryotic cells to minimize harm to human cells.
Unicellular vs. Multicellular
- Unicellular (Bacteria):
- Must perform all essential life functions independently.
- Rapid reproduction allows for adaptation to diverse environments.
- Exhibit a wide range of metabolic capabilities.
- Multicellular (Eukaryotes):
- Cells specialize in specific functions, leading to division of labor.
- Coordination and communication between cells enable complex organismal functions.
- Developmental processes lead to cell differentiation and tissue formation.
Gene Transformation
- Advantages in Bacteria:
- Asexual reproduction facilitates rapid dissemination of advantageous traits.
- Horizontal gene transfer mechanisms (conjugation, transduction, transformation) increase genetic diversity and promote bacterial adaptation.
Prokaryotic Cell Structure and Function
- Prokaryotic Cell Structure:
- Cytoplasm contains ribosomes, enzymes, and nucleoid.
- Lack membrane-bound organelles.
- Capsule or slime layer surrounds some bacteria, aiding in protection and attachment.
- Some possess endospores, allowing survival in harsh conditions.
- Metabolism:
- Diverse metabolic pathways enable adaptation to various environments.
- Anaerobic and aerobic respiration, fermentation, and photosynthesis are common metabolic processes.
- Bacterial Growth:
- Binary fission is the primary mode of reproduction.
- Generation time varies among species and environmental conditions.
- Ecological Roles:
- Bacteria play crucial roles in nutrient cycling, decomposition, and symbiotic relationships.
Some bacteria are pathogens, causing diseases in plants, animals, and humans.