Stem Cell Reprogramming and Therapeutic Applications
Reprogramming Adult Cells to Pluripotency
Can terminally differentiated adult cells be reprogrammed to become pluripotent?
- Yes, this was demonstrated by semi-starving cells from a mammary cell donor, effectively reversing their maturation (dedifferentiation). The process involved taking an enucleated egg from a donor and fusing it with the mammary cell. The resulting cell was cultured to form an embryo, which was then implanted into a surrogate sheep. This sheep carried the embryo to term and gave
Drug Elimination, Mechanisms, and Therapeutic Levels
Renal Drug Elimination
Renal elimination is the primary route for drug removal from the body, involving several kidney excretion mechanisms.
Enterohepatic Recirculation (Biliary Excretion)
Some drugs are excreted through bile, which is released into the duodenum to aid fat emulsification during digestion.
Pulmonary Elimination
The respiratory mucosa can eliminate volatile substances like anesthetics and alcohol.
Elimination Through Glands
Elimination can occur through glands, notably the mammary gland,
Read MoreUnderstanding Biomembranes: Structure, Function, and Properties
Understanding Biomembranes
A necessary condition for life is the existence of a barrier separating cell contents from the environment, since the cells must retain an internal environment appropriate to carry out the numerous chemical reactions necessary to sustain life. Biomembranes are the place where many chemical reactions take place. They regulate the entry and exit of materials from the cell. Some membranes are selectively permeable barriers that regulate the flow of molecules inwards and outwards,
Read MoreSmall and Large Intestine: Composition, Function, and Models
Composition and Function of the Small Intestine
Digestion within the small intestine produces a mixture of disaccharides, peptides, fatty acids, and monoglycerides. The final digestion and absorption of these substances occur in the villi, which line the inner surface of the small intestine.
- The crypts at the base of the villi contain stem cells that continuously divide by mitosis, producing:
- More stem cells
- Cells that migrate up the surface of the villus while differentiating
- Columnar epithelial cells
Antioxidants and Oxidative Stress in Exercise
Sources of Oxidative Stress
- Uncoupling of the electron transport chain: 2-5% of the total electron flux escapes the respiratory chain to make O2-. Exercise increases ATP demand. O2 uptake can increase 20-fold. O2 flux through active muscle may increase 100-fold. So there is an increased possibility.
- Ischemia-reperfusion: Intense exercise can lead to tissue hypoxia. Hypoxia promotes an excess of electron donors, NADH. Xanthine oxidase. After a period of ischemia, an increased neutrophil infiltration
Human Digestion: Enzymes, Absorption, and Assimilation
Why is Digestion of Large Food Molecules Essential?
Firstly, the food we eat is made up of many compounds produced by other organisms. Not all of these are suitable for human tissues. Therefore, these compounds have to be broken down and reassembled so that our bodies can use them. Secondly, food molecules must be small enough to be absorbed by the villi in the intestine through diffusion, facilitated diffusion, or active transport. Consequently, large food molecules need to be broken down into smaller
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